Monoquant provides the following services for external samples:
For samples from Australia, EDTA blood or marrow is preferred, as quality control is optimal. For samples from overseas, dried-down extracted DNA is preferred. It is usually possible to isolate and sequence the breakpoint using DNA extracted from blood or bone marrow smears on slides, but this is not always the case owing to DNA degradation. For this reason, two slides should be sent.
The charges for determination of BCR-ABL sequences are:
|2-3 sequences||A$160 per sequence|
|4-5 sequences||A$140 per sequence|
|6-10 sequences||A$120 per sequence|
|> 10 sequences||A$110 per sequence|
This charge includes the charge for confirmation of the sequence by amplifying across it.
Monoquant has developed a library of BCR and ABL primers which have been pretested for amplification efficiency and absence of non-specific amplification. The charge for one primer pair plus sufficient probe for 50 reactions is A$100. If two primer pairs are required in order to perform nested PCR for quantification of MRD below 10-4, then the charge for 2 primer pairs and probe is A$150.